Figure 2.

Biofilm formation inhibition of immunized rats' serum or saliva and its correlation with rPAc-specific antibody and total caries scores. 100 µl BHI diluted rat serum or saliva were mixed with 100 µl BHI diluted S. mutans and incubated for 16 h. The biofilm formation was quantified by measuring the extracted crystal violet stained to plate adherent bacteria and derivatives at 570 nm. The inhibitory effects of 20-fold diluted rat serums (A) and 5-fold diluted saliva (B) form immunized rats that challenged with S. mutans were shown. Data are represented as mean ± SE for 6 samples of one representative experiment that repeated 3 times (*, p < 0.05; **, p < 0.01; ***, p < 0.001). (C and D), Correlation between biofilm formation and rPAc-specific rat serum IgG or saliva IgA. (E and F), Correlation between caries scores and biofilm formation with the presence of rat serum or saliva. Data are analyzed by Graphpad Prism 5. Dotted lines represent the 95% confidence intervals. The correlation coefficients (r) and p values are also shown.