Figure 4.

The inhibitory of mice serum or saliva on S. mutans biofilm formation and the correlation with rPAc-specific antibody. 100 µl BHI diluted mice serum or saliva were mixed with 100 µl BHI diluted S. mutans and incubated for 16 h. The biofilm formation was quantified by measuring the extracted crystal violet stained to plate adherent bacteria and derivatives at 570 nm. (A and B), Inhibitory effects of 20-fold diluted rat serums and 5-fold diluted saliva form immunized mice. Data are represented as mean ± SE for 6 samples of one representative experiment that repeated 3 times (***, p <0.001). (C and D), Correlation between biofilm formation and serum rPAc-specific IgG or saliva rPAc-specific IgA. Data are analyzed by Graphpad Prism 5. Dotted lines represent the 95% confidence intervals. The correlation coefficients (r) and p values are also shown.