Figure 8.

Lung histology 24 h after bacterial instillation. Saline, pooled low titer hu-IgG, pooled high titer hu-IgG, or anti-PcrV rab-IgG was intravenously administered 1 h prior to tracheal instillation of a lethal dose (1.5 × 10⁶ CFU/mouse) of P. aeruginosa PA103. A. no bacterial control (tracheal instillation of saline solution alone). B. intravenously pretreated with saline. C. intravenously pretreated with pooled low titer hu-IgG. D. intravenously pretreated with pooled high titer hu-IgG. E. intravenously pretreated with anti-PcrV rab-IgG. Mouse lungs were fixed 24 h after bacterial instillation. Hematoxylin-eosin staining was conducted after 10% formaldehyde fixation and paraffin embedding. Magnification, 100× and 400×.