Fig. 1.
Crystal structure of the Aurora-A/N-Myc complex. (A) Domain structure of N-Myc with conserved regions indicated as boxes and the AIR marked with a red line. (B) Quantification of the binding affinity between the Aurora-A kinase domain and the N-Myc AIR by competition AlphaScreen assay. Data represent the mean of three experiments ± SD. (C) Crystal structure of the Aurora-AC290A/C393A catalytic domain (AurA, green cartoon) in complex with N-Myc residues 61–89 (N-Myc, red cartoon). (D) Magnified view of the structure with key residues shown as sticks.