Fig. S3.

AtGH3.5-overexpressing plants. (A) Comparison of 1-mo-old A. thaliana wild-type Col-0 rosettes (Left) and 1-mo-old 35S:Atgh3.5 rosettes for overexpressing lines OE1–4 (Right). (B) Immunoblot analysis of AtGH3.5::FLAG (transgenic lines OE1–4) expression in transgenic A. thaliana plants. Wild-type A. thaliana Col-0 is included as a negative control. The molecular weight (MW) ladder is shown. Equal amounts (20 μg) of total soluble protein extracted from 1-mo-old leaf tissues were separated by SDS/PAGE, electrotransferred, and probed with anti-FLAG M2 antibody to detect FLAG-tagged proteins.