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AtGH3.5 acyl acid specificity
| Substrate | kcat, min−1 | Km, μM | kcat/Km, M−1⋅s−1 |
| IAA | 14.5 ± 1.4 | 770 ± 110 | 314 |
| PAA | 28.0 ± 2.0 | 1,840 ± 233 | 253 |
| NAA | 2.3 ± 0.3 | 220 ± 70 | 174 |
| SA | 0.8 ± 0.1 | 700 ± 130 | 19 |
| BA | 16.0 ± 0.8 | 790 ± 60 | 338 |
| 4-HBA | 2.5 ± 0.3 | 300 ± 120 | 139 |
Average values ± SD (n = 3–5) are shown. Assays were performed as described with fixed concentrations of ATP (1 mM) and Asp (5 mM) and with varied concentrations of acyl acid substrates (up to 15 mM depending on the Km value).
