Table 1.
STD fitting parameters
| Regions | Residues | Isoflurane-CF3 | Isoflurane-CF2 | ||||
| STDmax, % | ksat, s–1 | σ×100, s–1 | STDmax, % | ksat, s–1 | σ×100, s–1 | ||
| Linker | 129 | 26.3 ± 0.6 | 2.7 ± 0.5 | 70 ± 13 | 20.5 ± 1.1 | 2.4 ± 1.1 | 49 ± 23 |
| 150 | 6.3 ± 0.3 | 1.8 ± 0.4 | 11 ± 2.6 | 6.3 ± 0.4 | 2.1 ± 0.8 | 13 ± 5.1 | |
| Extracellular interface | 179 | 0.52 ± 0.1 | 1.5 ± 0.9 | 0.8 ± 0.5 | 2.5 ± 0.1 | 1.8 ± 0.4 | 4.4 ± 1.0 |
| 208 | 7.5 ± 0.4 | 1.6 ± 0.4 | 12 ± 3.1 | 24.0 ± 1.2 | 1.1 ± 0.3 | 26 ± 7.3 | |
| Filter pore | 189 | 6.7 ± 0.3 | 2.0 ± 0.5 | 13 ± 3.4 | 14.0 ± 0.3 | 2.3 ± 0.3 | 32 ± 4.3 |
| PHE 224/227 | 1.3 ± 0.2 | 1.3 ± 1.0 | 1.6 ± 1.3 | 1.7 ± 0.3 | 1.4 ± 1.4 | 2.4 ± 2.4 | |
Fitting results from Eq. 1 for the 19F-NMR STD data in Fig. 4 covering several structural regions of NaChBac, including the linker region (S129 and L150), the extracellular interface (L179 and S208), the filter region (T189), and PHE residues in the pore (F224/F227) and other regions of NaChBac (Fig. 2). σ = ksat × STDmax.