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The observed promiscuous activity of OxsA is likely due to flexibility in the His75 region of OxsA. B-factors are plotted on a scale of 20–65 Å2 with increasing values colored blue-white-red. Although His75 should favor the smaller four-membered ring, the flexibility in the His75 region that may allow for active site rearrangements, and thus the observed activity on deoxyribonucleotide molecules.
