. 2016 Nov 11;113(48):13744–13749. doi: 10.1073/pnas.1610917113

Table S4.

EF-Tu−free codon selection by Glu-tRNA^Glu and Phe-tRNA^Phe

Glu-tRNA^Glu								Phe-tRNA^Phe
Mutants	GAA Cognate k_cat/K_m, M⁻¹⋅s⁻¹	GAU		GGA		GAC		UUC Cognate k_cat/K_m, M⁻¹⋅s⁻¹	CUC		UCC		UUA
Mutants	GAA Cognate k_cat/K_m, M⁻¹⋅s⁻¹	k_cat/K_m, M⁻¹⋅s⁻¹	A_tRNA	k_cat/K_m, M⁻¹⋅s⁻¹	A_tRNA	k_cat/K_m, M⁻¹⋅s⁻¹	A_tRNA	UUC Cognate k_cat/K_m, M⁻¹⋅s⁻¹	k_cat/K_m, M⁻¹⋅s⁻¹	A_tRNA	k_cat/K_m, M⁻¹⋅s⁻¹	A_tRNA	k_cat/K_m, M⁻¹⋅s⁻¹	A_tRNA
WT	103 ± 12	7.9 ± 1.4	13 ± 3	4.1 ± 0.7	25 ± 5	5.0 ± 0.2	21 ± 3	189 ± 9	4.8 ± 0.7	39 ± 6	18.6 ± 0.3	10 ± 0.5	15 ± 0.4	13 ± 0.7
T1	134 ± 13	11.9 ± 0.5	11 ± 1	4.7 ± 0.2	29 ± 3	5.3 ± 0.4	26 ± 3	338 ± 10	7.1 ± 0.4	47 ± 3	22.2 ± 0.2	15 ± 0.5	21 ± 0.2	16 ± 0.5
T2	111 ± 18	7.0 ± 1.0	16 ± 3	5.6 ± 0.5	20 ± 4	4.2 ± 0.05	26 ± 4	346 ± 31	12.2 ± 0.4	28 ± 3	23.2 ± 0.4	15 ± 1.4	20 ± 0.5	17 ± 2
W1	82 ± 4	8.7 ± 1.3	10 ± 1	2.9 ± 1.0	28 ± 10	8.1 ± 1.3	10 ± 2	239 ± 15	5.1 ± 0.2	46 ± 3	22.9 ± 0.2	10 ± 0.7	19 ± 0.4	12 ± 0.8
W2	59 ± 5	4.0 ± 0.5	15 ± 2	2.2 ± 0.2	26 ± 3	4.1 ± 0.3	15 ± 2

The k_cat/K_m values were determined from titrations with aa-tRNA in excess over 70S ribosomes (Materials and Methods and SI Materials and Methods). Data represent weighted averages from at least two independent experiments ± propagated SD.