Fig. 1.

A disease phenotype in Ae. aegypti infected with recombinant SINVs expressing heterologous VSR proteins. (A) Schematic of a recombinant SINV expressing a VSR from an engineered subgenomic promoter (SG2). (B) Survival of mosquitoes after infection with recombinant SINVs expressing well-known VSR proteins (red triangles) or with viruses containing nontranslatable versions of the VSR sequences (black circles). Survival curves represent cohorts of ≥40 adult female mosquitoes injected with 500 pfu of virus. Significance was determined using the Mantel–Cox log-rank test.