The functional activity of purified antibodies from HIV infected treated (Tx), untreated (Untx), or elite controllers (EC), Vax004 vaccinees (VAX004), and VAX004 placebo recipients (Placebo) was assessed across a suite of cell-based assays of antibody effector function including ADCC activity (BVADCC, GTL ADCC, RFADCC, LUC ADCC), ADCVI, and phagocytic activity (Virion Phagocytosis, ADCP). Replicates of polyclonal IVIG, HIVIG, and HIVIG-C were used as negative and positive controls and to assess assay reproducibility, and were evaluated at a different concentration (gray shaded region). AUC denotes area under the titration curve. Activity magnitudes (y-axis scales) are described in the materials and methods and should not be compared across assays.