(A) Freshly isolated HAMs were adhered to coverslips, fixed with PFA, left intact or permeabilized with methanol and labeled with anti-CD36 Ab followed by AlexaFluor488. Scale bar, 50 μm. (B, C) MDMs were left untreated or were exposed to SP-A (10 μg/mL), Survanta (100 μg/mL) or both up to 96h. Monolayers were lysed with TriZOL. CD36 transcript levels (B) or SR-A transcript levels (C) were determined via qRT-PCR. (D, E) MDMs on coverslips were left untreated or exposed to Survanta (100 μg/mL) for 96h. Monolayers were fixed with PFA and were (D) permeabilized by methanol or (E) left intact and labeled with anti-CD36 Ab. Scale bars, 50 μm. (F) Fold change in CD36 MFI in Survanta-exposed MDMs relative to untreated cells 96h after Survanta exposure. Experiments were conducted in duplicate from 3 (A, C–F) independent donors or in triplicate from 4 (B) independent donors (mean ± SEM).