Fig 5. Comparison of the image processing methods: Eqs (2) and (3).

Consecutive frames were taken from a video of a well with 4x10⁴ parasites in LIT medium and benznidazole 0.3125μg/mL and frames 100 to 180 were processed with ImageJ using either Eqs (2) or (3); consecutive frames were taken from a video of a well with LIT medium and benznidazole 0.3125μg/mL without parasites and frames 100 to 180 were processed with ImageJ and Eq (3); consecutive frames were taken from a video of a well with 4x10⁴ parasites in LIT medium and benznidazole 0.3125μg/mL and frames 100 to 200 were processed with R/SAGA and Eq (3); consecutive frames were taken from a video of a well with LIT medium and benznidazole 0.3125μg/mL and without parasites and frames 100 to 200 were processed with R/SAGA and Eq (3).