Table 2. . Nanostructured lipid carriers fabricated using emulsification and low-temperature solidification technique.

Active ingredient	Solid lipid	Liquid lipid	Surfactants	Size (nm)	Research highlights	Ref.
Baicalin	Glycerol monostearate	Medium chain triglyceride	Soya lecithin	244.7	1.9-fold improvement in AUC and 1.7-fold improvement in mean residence time in case of drug-loaded NLCs in comparison to drug suspension	[23]
Biochanin A	Glycerol monostearate	Medium chain triglyceride	Soya lecithin, Tween 80	148.5 ± 2.88	Higher AUC value of drug-loaded PEGylated NLCs in comparison to drug suspension Biochanin-loaded PEGylated NLCs showed higher in vitro cytotoxicity against MCF-7 cell line compared with drug suspension	[25]
Etoposide	Glyceryl monostearate	Soyabean oil	Soya lecithin	91.2–125.9	Higher cytotoxicity of drug-loaded NLCs against human epithelial-like lung carcinoma cell	[26]
Silymarin	Glycerol monostearate	Oleic acid	Tween 80	223.73 ± 43.39	In vivo study revealed high accumulation of drug in liver after encapsulation in NLCs	[15]

AUC: Area under curve; NLC: Nanostructured lipid carrier.