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. 2016 Nov 22;5:e19662. doi: 10.7554/eLife.19662

Fly stock

Description

w1118

Control strain

;;UAS-dome △Cyt 2.3

Overexpression of a dominant negative form of the receptor domeless.

;UAS-dPIAS/cyO;

Overexpression of the JAK/STAT pathway negative regulator dPIAS.

;if/cyO;Hs-Gal4

Heat shock-inducible, ubiquitous driver line.

w1118;;Hml△-Gal4,

UAS-2xeGFP,

Tub-Gal80ts/TM6C,Sb1;

Temperature-sensitive, haemocyte-specific driver line. Haemocytes are labelled with GFP.

w1118;;crq-Gal4, UAS-2xmRFP, Tub-Gal80ts/TM6C.Sb1

Temperature-sensitive, haemocyte-specific driver line. Haemocytes are labelled with RFP.

w;;UAS-rpr/TM3.Sb1

Overexpression line for pro-apoptotic protein reaper.

;;r4-Gal4/TM6C.Sb1

Constitutive fat body-specific driver line.

;;Stat92-dGFP

Destabilised GFP expression under the control of a STAT response element. Bloomington

stock centre number: 21699

LPP-Gal4-UAS-Myr-td-Tom

Fat body line, made from LPP-Gal4 (FRT-LPPGal4-G-FRT/Tm3,Zit(Sb) from Pierre Leopold;

10XUAS-IVS-myr::tdTomato from Bloomington (BL32221).

w1118;Tub-Gal80ts;Tub-Gal4/TM6C.Sb1

Temperature-sensitive, ubiquitous driver line.

;UAS-Shark IR;

(Shark R2 RNAi)

National Institute of Genetics stock number: 18247 R-2

;UAS-Shark IR;

(Shark R3 RNAi)

National Institute of Genetics stock number: 18247 R-3

;UAS-Shark IR;

(Shark Fr RNAi)

Interfering RNA for knockdown of shark. Kindly donated by Marc Freeman.

w;c564-Gal4;Tub-Gal80ts

Temperature-sensitive fat body-specific driver line.

;UAS-Src42A IR V10708RNAi

Interfering RNA for knockdown of Src42A.

;;UAS-Src42ADN

Overexpression of a dominant negative form of Src42A.

UAS-Src42ACA

Bloomington stock centre number: 6410. The DNA sequence encoding the constitutively

active form of Src42A (Src42ACA) has an amino acid substitution of Tyr511 to Phe. Tyr511

corresponds to the inhibitory C-terminal Tyr of Src.

UAS-Nox IR (753)

VDRC ID: 100753

UAS-Nox IR (559)

VDRC ID: 102559

UAS-Duox IR (692)

Bloomington stock centre number: 32903

UAS-Duox IR (934)

Bloomington stock centre number: 33975

;;drprΔ5

Null mutant for the receptor Draper. Kindly donated by Marc Freeman.

UAS-drpr IR (4833)

VDRC ID: 4833

UAS-drpr IR (4830)

VDRC ID: 4830

UAS-drpr IR (27084)

VDRC ID: 27084

UAS-drpr IR (27086)

VDRC ID: 27086

;FB-Gal4;

Constitutive fat body-specific driver line.

UAS-Sod

Bloomington stock centre number: 24750