Figure 4.

MiR-1247 targeted STMN1 in A549 and H1299 cells.

Notes: (A) Luciferase reporter assay was used to determine whether STMN1 was the direct target of miR-1247 in A549 and H1299 cells. A normal and a mutated 3′-UTR of STMN1 were subcloned into the psi-CHECK2/Neo reporter vector. Psi-CHECK2/Neo-STMN1-3′UTR or psi-CHECK2/Neo-mut STMN1-3′UTR plus miR-1247 mimics or miR-1247 inhibitor were co-transfected. The fluorescence intensities were detected to mirror the capacity for direct binding. (B) The mRNA expression of STMN1 was detected in A549 and H1299 cells with miR-1247 upregulated and silenced with pre-miR and anti-miR. The expression of STMN1 protein was detected by Western blot in A549 (C) and H1299 (D) miR-1247-upregulated and -silenced cells. (E) Bars represent mean values ± standard deviation of (C) and (D). ***P<0.001; **P<0.01; *P<0.05, N=3.

Abbreviation: Con, control.