Figure 1.
Sample processing of retroillumination photography images of Fuchs corneal dystrophy. After the original image was uploaded to Image J Software (far left), subsequent steps included conversion to 8 bit-grayscale (left), subtraction of background noise to better visualize guttae (center), application of Find maxima function to overlay each gutta with a point (right), and refinement by magnifying a selected area to ensure an appropriate ratio of points to guttae (far right).