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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Cell Signal. 2016 Oct 17;29:78–83. doi: 10.1016/j.cellsig.2016.10.006

Figure 1. Development of an analog sensitive kinase system for eEF2K.

Figure 1

(A) Active site of Src, indicating gatekeeper residues. Val 323 was initially considered, but Thr 338 became the successful gatekeeper residue. (B) Homology model of eEF2K active site, based on the structure of MHCK. (C) Purification of recombinant full-length eEF2K protein in bacteria, showing the coomassie-stained gel of the final protein. (D) Analysis of eEF2K wild type and mutant usage of ATP analogs for autophosphorylation and transphosphorylation, using MBP as a substrate. The reactions were performed with ATPγS and blotted with MAb 51–8.