Skip to main content
. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Cell Signal. 2016 Oct 17;29:78–83. doi: 10.1016/j.cellsig.2016.10.006

Figure 3. Discovery and validation of two new substrates of eEF2K: IGBP1 (alpha 4) and NDRG1.

Figure 3

Wild type and mutant IGBP1 and NDRG1 proteins were purified from HEK293T cells and phosphorylated using 32P-γ-ATP. The samples were analyzed by SDS-PAGE followed by autoradiography. Samples were also analyzed by western blot for loading prior to addition of the 32P-γ-ATP.