Table 1. Selected phosphorylated hits identified from eEF2K mass spectrometry analysis.

Example peptides discovered with the phosphosite shown in bold. We note that for several of proteins multiple sites were identified. For the full list, see Supplementary Table 1. These substrates are ones that were identified as phosphopeptides and were enriched in the AS kinase samples relative to the lysate only controls.

Symbol	Name	Phosphopeptide
EEF2K	Eukaryotic elongation factor 2 kinase	DSGYPSEKRGE
EEF2	Elongation factor 2	GETRFTDTRKD
HSPD1	Heat shock protein 60	TKDGVTVAKSI
PTMA	Prothymosin alpha	TSSEITTKDLK
HNRNPA1	Heterogeneous nuclear ribonucleoprotein A1	VSREDSQRPGA
SRC8	Src substrate cortactin	EYQGKTEKHAS
NDRG1	N-myc downstream-regulated gene 1 protein	TSLDGTRSRSH
MAP4	Microtubule-associated protein 4	TEAAATTRKPE
HN1L	Hematological and neurological expressed 1- like protein	FGSPVTATSRL
IGBP1 (alpha4)	Immunoglobulin-binding protein 1	EDDEQTLHRAR
SARNP	SAP domain-containing ribonucleoprotein	GTTEDTEAKKR
SAFB2	Scaffold attachment factor B2	VISVKTTSRSK
PCNP	PEST proteolytic signal-containing nuclear protein	AIGSQTTKKAS
PRP31	U4/U6 small nuclear ribonucleoprotein Prp31	ERLGLTEIRKQ