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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: J Immunother. 2017 Jan;40(1):11–20. doi: 10.1097/CJI.0000000000000145

Figure 1. Design of polymer-conjugated CpG ODN2216 (PG-CpG).

Figure 1

(A) Structures of PG-CpG and PG-CpG-NIR813. (B) Purity of PG-CpG. Free CpG (CpG-3′-NH2), CpG-3′-acetylthioacetamide (CpG-3′-SATA), and purified PG-CpG were loaded onto 15% polyacrylamide-urea gel and stained with SYBR Gold stain. (C and D) HPLC analysis of 111In-labeled CpG (C) and 111In-labeled PG-CpG (D). The overlay of UV absorbance peak and radioactivity peak confirmed radiolabeling of 111In onto CpG and PG-CpG. The radiochemical purities of 111In-CpG and 111In-PG-CpG were 97% and 95%, respectively. A.U., arbitrary units.