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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Cell Signal. 2016 Sep 23;29:1–11. doi: 10.1016/j.cellsig.2016.09.005

Figure 7. Effect of EB1 knockdown on HGF-mediated regulation of actin remodeling.

Figure 7

Human pulmonary EC were transfected with EB1-specific or non-specific siRNA. A - Cells grown on glass coverslips were stimulated with HGF (50 ng/ml, 10 min). Intracellular redistribution of p21-Arc was examined by immunofluorescence staining with corresponding antibody. Shown are representative results of three independent experiments. B – Control and EB1-depleted EC were used for co-immunoprecipitation assays with IQGAP1 antibody followed by western blot detection of WAVE and p21-Arc. Bar graphs depict quantitative densitometry analysis of immunoblotting data. Results are represented as mean ± SD; *P<0.05; n=4.