TABLE 2.
Primary Antibodies
| Antigen | Immunogen | Source1, host species, catalog number, research resource identified |
Dilution2 |
|---|---|---|---|
| Neuron specific nuclear protein (NeuN) |
Purified cell nuclei from mouse brain |
EMD Millipore, mouse monoclonal A60, Cat# MAB3777, RRID: AB_2314890 |
1:200 (IHC) |
| Glial fibrillary acidic protein (GFAP) |
Purified GFAP from porcine spinal cord. |
EMD Millpore, mouse monoclonal GA5, Cat# MAB360, RRID: AB_212974 |
1:200 (IHC, IF); 1:1,000 (IB) |
| Slit homolog 2 protein precursor (Slit2) |
Synthetic peptide corresponding to residues in Human Slit2 |
Abcam, rabbit monoclonal, Cat# ab134166, RRID: YG100906C |
1:100 (IF); 1:1,000 (IB) |
| Myelin basic protein (MBP) |
Purified human myelin basic protein. |
QED Bioscience, mouse, monoclonal, Cat# 24201, RRID: AB_129975 |
1:200 (IHC); 1:500 (IB) |
| 2',3'-Cyclic-nucleotide 3'-phosphodiesterase (CNPase) |
Human cerebellum tissue |
Biolegend, mouse monoclonal SMI 91, Cat# 836401, RRID: AB_2565361 |
1:200 (IHC); 1:1,000 (IB) |
| Synaptophysin | Rat retina synaptosomes | Sigma-Aldrich, mouse monoclonal SVP 38, Cat# S5768, RRID: AB_477523 |
1:200 (IHC); 1:1,000 (IB) |
| βIII-tubulin | Microtubules derived from rat brain |
Biolegend, mouse, monoclonal, Clone TUJ1, Cat# 801201, RID: AB_2564639 |
1:2,000 (IB) |
| Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) |
Rabbit GAPDH | Sigma-Aldrich, mouse, monoclonal, Clone GAPDH-71.1, Cat# G8795, RRID: AB_1078991 |
1:5,000 (IB) |
| Myelin-associated glycoprotein (MAG) |
Human MAG | Kind gift of Dr. Norman Latov, Cornell University, Ithaca, NY, Clone GEN-S3 |
1:500 (IB) |
| Nogo-A | Amino acids 701–1000 of Nogo A of human origin |
Santa Cruz Biotechnology, rabbit, polyclonal Clone H-300, Cat# sc25660, RRID: AB_2285559 |
1:500 (IB) |
| Oligodendrocyte myelin glycoprotein (OMgp) |
Amino acids 195–416 of human OMgp |
Santa Cruz Biotechnology, mouse, monoclonal, Clone E-8, Cat# sc271704, RRID: AB_10707816 |
1:500 (IB) |
1
Commercial supplier locations: EMD Millipore, Billerica, MA; Abcam, Cambridge, MA; QED Bioscience, San Diego, CA; BioLegend, San Diego, CA; Santa Cruz Biotechnology, Dallas, TX; and Sigma-Aldrich, St. Louis, MO.
2
IHC, immunohistochemistry; IF, immunofluorescence; IB, immunoblotting