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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: J Comp Neurol. 2016 Jul 8;525(2):291–301. doi: 10.1002/cne.24064

TABLE 2.

Primary Antibodies

Antigen Immunogen Source1, host species, catalog
number, research resource identified
Dilution2
Neuron specific
nuclear protein (NeuN)
Purified cell nuclei from
mouse brain
EMD Millipore, mouse monoclonal A60,
Cat# MAB3777, RRID: AB_2314890
1:200 (IHC)
Glial fibrillary acidic
protein (GFAP)
Purified GFAP from
porcine spinal cord.
EMD Millpore, mouse monoclonal GA5,
Cat# MAB360, RRID: AB_212974
1:200 (IHC,
IF); 1:1,000
(IB)
Slit homolog 2 protein
precursor (Slit2)
Synthetic peptide
corresponding to
residues in Human Slit2
Abcam, rabbit monoclonal,
Cat# ab134166, RRID: YG100906C
1:100 (IF);
1:1,000 (IB)
Myelin basic protein
(MBP)
Purified human myelin
basic protein.
QED Bioscience, mouse, monoclonal,
Cat# 24201, RRID: AB_129975
1:200 (IHC);
1:500 (IB)
2',3'-Cyclic-nucleotide
3'-phosphodiesterase
(CNPase)
Human cerebellum
tissue
Biolegend, mouse monoclonal SMI 91,
Cat# 836401, RRID: AB_2565361
1:200 (IHC);
1:1,000 (IB)
Synaptophysin Rat retina synaptosomes Sigma-Aldrich, mouse monoclonal SVP
38, Cat# S5768, RRID: AB_477523
1:200 (IHC);
1:1,000 (IB)
βIII-tubulin Microtubules derived
from rat brain
Biolegend, mouse, monoclonal, Clone
TUJ1, Cat# 801201, RID: AB_2564639
1:2,000 (IB)
Glyceraldehyde 3
phosphate
dehydrogenase
(GAPDH)
Rabbit GAPDH Sigma-Aldrich, mouse, monoclonal,
Clone GAPDH-71.1, Cat# G8795,
RRID: AB_1078991
1:5,000 (IB)
Myelin-associated
glycoprotein (MAG)
Human MAG Kind gift of Dr. Norman Latov, Cornell
University, Ithaca, NY, Clone GEN-S3
1:500 (IB)
Nogo-A Amino acids 701–1000 of
Nogo A of human origin
Santa Cruz Biotechnology, rabbit,
polyclonal Clone H-300, Cat# sc25660,
RRID: AB_2285559
1:500 (IB)
Oligodendrocyte
myelin glycoprotein
(OMgp)
Amino acids 195–416 of
human OMgp
Santa Cruz Biotechnology, mouse,
monoclonal, Clone E-8, Cat# sc271704,
RRID: AB_10707816
1:500 (IB)
1

Commercial supplier locations: EMD Millipore, Billerica, MA; Abcam, Cambridge, MA; QED Bioscience, San Diego, CA; BioLegend, San Diego, CA; Santa Cruz Biotechnology, Dallas, TX; and Sigma-Aldrich, St. Louis, MO.

2

IHC, immunohistochemistry; IF, immunofluorescence; IB, immunoblotting