Table 1.

Primary antibodies used for immunohistochemistry

Antibody	Immunogen	Source/RRID	Dilution	Specificity
Rat anti-DN-cadherin	Exon 8 (a.a. residues 1210-1272) of Drosophila CadN gene	Developmental Studies Hybridoma Bank (DSHB), Iowa City, Iowa, DN-Ex #8; RRID:AB_2314331	1:10	Detected by Western Blot of DN-cadherin transfected S2 cells and immunolabeling of Drosophila embryos (Iwai et al., 1997)
Mouse anti-Neuroglian	18 N-terminal a.a. residues of the Drosophila Neuroglian gene	DSHB, BP104; RRID:AB_528402	1:10	Protein purification by immunoaffinity chromatography using the neuroglian antibody and microsequencing (Bieber et al., 1989)
Mouse anti-Neurotactin	1-280 aminoterminal a.a. residues of Drosophila Neurotactin gene	DSHB, BP106; RRID:AB_528404	1:10	Immunolabeling in embryos matches pattern fusion protein made from Neurotactin cDNA (Hortsch et al., 1990) and an additional monoclonal Neurotactin antibody (Piovant and Léna, 1988)
Mouse anti-Tyrosine hydroxylase	Full length tyrosine hydroxylase (TH) from rat PC12 cells; recognizes 34 kDa catalytic core	Immunostar, 22941; RRID:AB_572268	1:300	Immunolabeling of rat catecholamine neurons (TH required for synthesis catecholamine)