Across all nTS levels, Fos-LI (green circles) is significantly reduced in the nTS of P2X-dbl KO mice compared to WT. In contrast, in non-nTS areas displaying high levels of CGRP-immunoreactivity (magenta fibers), including the dorsomedial trigeminal nucleus (DMSp5), spinal trigeminal tract (SP5), and the nucleus Ambiguus (Nuc. Amb.), Fos-LI density is similar between P2X WT and P2X-dblKO mice.