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. 2016 Dec 6;7:1866. doi: 10.3389/fpls.2016.01866

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Copyright © 2016 An, Ma, Yan, Zhou, Liu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Molecular and phenotypic characterization of Arabidopsis CBF3 overexpressing cassava. (A) Transgene integration pattern in wild-type (WT) and transgenic (OE) lines by Southern analysis. (B) Quantitative analysis of the DREB1A/CBF3 expression in WT and three single-copied transgenic lines (OE-1, OE-3, and OE-5) by real-time RT-PCR. (C) Leaves collected from field-grown plants. (D) Plant status in the field by comparison of WT and OE-3 plants. (E) Plant height and internode length between WT and OE plants. Data are presented as mean ± SD of three independent assays; asterisks indicate significant differences compared with the WT, ^∗P < 0.05 or ^∗∗P < 0.01 by Student’s t-test.