Table 1.
Strains and plasmids used in this study.
| Strains and plasmids | Relevant characteristics | Sources |
|---|---|---|
| E. COLI | ||
| S17-1λpir | thi pro hsdR hsdM+ recA RP4-2-Tc::Mu-Km::Tn7λpir | Milton et al., 1992 |
| BL21 (DE3) | F−ompT hsdSB () gal dcm (DE3) | Novagen |
| S17_pDM4_exsA_A1F+A2R | S17 carrying pDM4_exsA_A1F+A2R | This study |
| S17_pDM4_exsC_A1F+A2R | S17 carrying pDM4_exsC_A1F+A2R | This study |
| S17_pDM4_exsD_A1F+A2R | S17 carrying pDM4_exsD_A1F+A2R | This study |
| S17_pDM4_exsE_A1F+A2R | S17 carrying pDM4_exsE_A1F+A2R | This study |
| S17_pMMB207_exsA_His | S17 carrying pMMB207_exsA_His | This study |
| S17_pMMB207_exsC_His | S17 carrying pMMB207_exsC_His | This study |
| S17_pMMB207_exsD_His | S17 carrying pMMB207_exsD_His | This study |
| S17_pMMB207_exsE_His | S17 carrying pMMB207_exsE_His | This study |
| S17_pDM4_exsA_HA_Insertion | S17 carrying pDM4_exsA_HA_Insertion | This study |
| BL21_pACYCDuet-1_exsA | BL21 carrying pACYCDuet-1_exsA | This study |
| BL21_pACYCDuet-1_exsC | BL21 carrying pACYCDuet-1_exsA | This study |
| BL21_pACYCDuet-1_exsD | BL21 carrying pACYCDuet-1_exsD | This study |
| BL21_pACYCDuet-1_exsE | BL21 carrying pACYCDuet-1_exsE | This study |
| V. ALGINOLYTICUS | ||
| ZJO | Opaque variant of wild strain ZJ51; isolated from diseased grouper fish off the Southern China coast; Apr | Chen et al., 2009 |
| ΔvscC:pexsE | In trans complementation of exsE in a T3SS dysfunctional strain (ΔvscC); Apr | Zhao et al., 2010 |
| ΔexsA | exsA deletion mutant; Apr | This study |
| ΔexsA: pexsA | ΔexsA complemented in trans with wild-type exsA gene located in the plasmid of pMMB207; Apr | This study |
| ZJO: pexsA | Wild-type strain complemented in trans with wild-type exsA gene located in the plasmid of pMMB207; Apr | This study |
| ΔexsC | exsC deletion mutant; Apr | This study |
| ΔexsC: pexsC | ΔexsC complemented in trans with wild-type exsC gene located in the plasmid of pMMB207; Apr | This study |
| ZJO: pexsC | Wild-type strain complemented in trans with wild-type exsC gene located in the plasmid of pMMB207; Apr | This study |
| ΔexsD | exsD deletion mutant; Apr | This study |
| ΔexsD: pexsD | ΔexsD complemented in trans with wild-type exsD gene located in the plasmid of pMMB207; Apr | This study |
| ZJO: pexsD | Wild-type strain complemented in trans with wild-type exsD gene located in the plasmid of pMMB207; Apr | This study |
| ΔexsE | exsE deletion mutant; Apr | This study |
| ΔexsE: pexsE | ΔexsE complemented in trans with wild-type exsE gene located in the plasmid of pMMB207; Apr | This study |
| ZJO: pexsE | Wild-type strain complemented in trans with wild-type exsE gene located in the plasmid of pMMB207; Apr | This study |
| ZJO_ pDM4_exsA_HA_Insertion | Wild-type strain was incorporated a exsA gene with a HA tag at the 3′ terminus in the chromosome; AprCmr | This study |
| ΔexsC_ pDM4_exsA_HA_Insertion | ΔexsC mutant was incorporated a exsA gene with a HA tag at the 3′ terminus in the chromosome; AprCmr | This study |
| V. PARAHAEMOLYTICUS | ||
| NY4 | Clinical isolate O3: K6 | Zhou et al., 2008 |
| PLASMIDS | ||
| pMMB207 | RSF1010 derivative, IncQ lacI q Cmr Ptac oriT | Morales et al., 1991 |
| pDM4 | A suicide vector with ori R6K sacB; Cmr | Milton et al., 1996 |
| pACYCDuet-1 | Prokaryotic expression vector with two MCS, each of which is preceded by a T7 promoter/lac operator; Cmr | Novagen |
| pDM4_exsA_A1F +A2R | Flanking region sequences of exsA cloned into pDM4 | This study |
| pDM4_exsC_A1F +A2R | Flanking region sequences of exsC cloned into pDM4 | This study |
| pDM4_exsD_A1F +A2R | Flanking region sequences of exsD cloned into pDM4 | This study |
| pDM4_exsE_A1F +A2R | Flanking region sequences of exsE cloned into pDM4 | This study |
| pDM4_exsA_HA_Insertion | exsA coding sequence and sequences for HA tag at the C-terminus cloned into pDM4 | This study |
| pMMB207_exsA_His | exsA coding sequence and sequences for 6 His amino acids at the C-terminus cloned into pMMB207 | This study |
| pMMB207_exsC_His | exsC coding sequence and sequences for 6 His amino acids at the C-terminus cloned into pMMB207 | This study |
| pMMB207_exsD_His | exsD coding sequence and sequences for 6 His amino acids at the C-terminus cloned into pMMB207 | This study |
| pMMB207_exsE_His | exsE coding sequence and sequences for 6 His amino acids at the C-terminus cloned into pMMB207 | This study |
| pACYCDuet-1_exsA | exsA coding sequence cloned into pACYCDuet-1 | This study |
| pACYCDuet-1_exsC | exsC coding sequence cloned into pACYCDuet-1 | This study |
| pACYCDuet-1_exsD | exsD coding sequence cloned into pACYCDuet-1 | This study |
| pACYCDuet-1_exsE | exsE coding sequence cloned into pACYCDuet-1 | This study |