Table 2.
Primers used in this study.
| Primer name | Sequences (5′-3′) |
|---|---|
| ExsA_A1_F | TGAAGATCTTATCTCGCTCCTTGAACAC |
| ExsA_A1_R | TAGCCACTTGTTTCTACCCTTCATTATTTTGA |
| ExsA_A2_F | AGGGTAGAAACAAGTGGCTATCGCGAAATGAA |
| ExsA_A2_R | AGCGTCGACCAGACGAGAGTTGATGTAGT |
| ExsA_int_F | TGTCGTTCACAATGGTCAG |
| ExsA_int_R | AGGCACATAATGGCATCAG |
| ExsC_A1_F | GTCAGAGCTCTCGACCCGTTAGGCTTCT |
| ExsC_A1_R | CTATGTCAGCGGTGTCTTTATGTCCAATGACA |
| ExsC_A2_F | TAAAGACACCGCTGACATAGGAATAGTCCC |
| ExsC_A2_R | GACTCTCGAGGAATAACCCAATAAAACC |
| ExsC_int_F | ACAATAACGCTTCCCACG |
| ExsC_int_R | TGTCAGCACGCCAAACTA |
| ExsD_A1_F | GTCAGAGCTCTGATGCCATTATGTGCCTAA |
| ExsD_A1_R | GAGGTGATTGTTTATGTTCGTCTCCGCAC |
| ExsD_A2_F | CGAACATAAACAATCACCTCAGCCAGAT |
| ExsD_A2_R | GACTCTCGAGCGTTCTTGTTCCAATAATGC |
| ExsD_int_F | GATAGCAGCACAATCACAAC |
| ExsD_int_R | GCACTTCCGAACACCAAT |
| ExsE_A1_F | GTCAGAGCTCTACATTCAGCCAACCATG |
| ExsE_A1_R | TTTATGTCCAGATATCACAATATAAGCAGG |
| ExsE_A2_F | TTGTGATATCTGGACATAAAGACACCTAAACTCTC |
| ExsE_A2_R | GACTCTCGAGCACTGCATCTAACGGAAA |
| ExsE_int_F | AGTTGCGGATCAAAGTCC |
| ExsE_int_R | ACACCAATTCATCGGTTC |
| ExsA-comp-F | AGGATAGAATTCATGGATGTGTCAGGCCAACTA |
| ExsA-comp-His-R | AGTTAGGGATCCTCAATGGTGATGGTGATGGTGTTTCGCGATAGCCACTTGA |
| ExsC-comp-F | AGGATAGAATTCATGTCAGCACGCCAAACTATC |
| ExsC-comp-His-R | AGTTAGGGATCCCTAATGGTGATGGTGATGGTGAACTCTCAAGTCTAAAGTTT |
| ExsD-comp-F | AGGATAGAATTCATGAAAAAGCAGCATTGGC |
| ExsD-comp-His-R | AGTTAGGGATCCTTAATGGTGATGGTGATGGTGGATCTGGCTGAGGTGATTGC |
| ExsE-comp-F | AGGATAGAATTCATGTCCAATGACATCCAATCCA |
| ExsE-comp-His-R | AGTTAGGGATCCTCAATGGTGATGGTGATGGTGGGAACGTTGAATTATCGCC |
| VscY_F | GGCGTGTTTACAAAGTGG |
| VscY_R | TGCCGAGTCAGGATGAAG |
| VseE_F | ATGAAGGCGAGACGAACA |
| VseE_R | GCACCCTAAATCCAACTGAC |
| 1687_F | ATGATTGTTGCCATCTACT |
| 1687_R | ACTCGGTTTATTACCTGAA |
| 1686_F | GCAAGCGGTGTTTGATAT |
| 1686_R | ATTGGTTACGCCACTTTT |
| VopB_F | AGAAGCGGGCGTAAATG |
| VopB_R | CACCACCAAACGTCACAAC |
| VopD_F | TCGGGTGTATTAGCGGGTGC |
| VopD_R | CTCGCCATTTCATTCTTGATTTCT |
| ExsA_F | AGCACTATGGCATTTCTC |
| ExsA_R | AACGACGACGGTAACTCT |
| ExsC_F | TAATCCAGTCGCCTAA |
| ExsC_R | CTCTATCGCTCTTTCTT |
| ExsD_F: | CGGAGTACACCTCTACAACC |
| ExsD_R | TCTTGAACCATTGCCATAC |
| ExsE_F | GCGTCATACTGCTTTCTG |
| ExsE_R | ACCAATTCATCGGTTCA |
| DnaK_F | TAAACCCTGACGAAGC |
| DanK_R | AGTCATCACGCCACCC |
| pACYC_ExsA_F | AGGATAGAATTCGATGGATGTGTCAGGCCAACTA |
| pACYC_ExsA_R | AGTTAGAAGCTTTCATTTCGCGATAGCCACTTGA |
| pACYC_ExsC_F | AGGATAGAATTCGATGTCAGCACGCCAAACTATC |
| pACYC_ExsC_R | AGTTAGAAGCTTCTAAACTCTCAAGTCTAAAGTTT |
| pACYC_ExsD_F | AGGATACGATCGATGAAAAAGCAGCATTGGC |
| pACYC_ExsD_R | AGTTAGCTCGAGTTAGGCGTAGTCAGGCACGTCGTAAGGATA GATCTGGCTGAGGTGATTGC |
| pACYC_ExsE_F | AGGATACGATCGATGTCCAATGACATCCAATCCA |
| pACYC_ExsE_R | AGTTAGCTCGAGTCAGGCGTAGTCAGGCACGTCGTAAGGATA GGAACGTTGAATTATCGCC |
| ExsA-insertion-F | AGGATAGAGCTCATGGATGTGTCAGGCCAACTA |
| ExsA-insertion-HA-R | AGTTAGTCTAGATCAGGCGTAGTCAGGCACGTCGTAAGGATATTTCGCGATAGCCACTTGA |