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. 2016 Dec 6;7:1808. doi: 10.3389/fpls.2016.01808

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Copyright © 2016 Deery, Rebetzke, Jimenez-Berni, James, Condon, Bovill, Hutchinson, Scarrow, Davy and Furbank.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Typical histogram of the frequency distribution of CT pixels from airborne thermography for a single 2 × 6 m experimental plot. Different histogram trimming methods used to minimize bias from background soil temperature are shown. M1, The mean of all pixels (no pixels discarded). M2, The mean of coolest 25th percentile remaining after discarding coolest 5th percentile, designed to extract leaf temperature only. M3, Designed to discard warm pixels from soil patches resulting from poor establishment or biomass sampling. Total pixels in histogram (i.e., M1): 671. Total pixels using M2: 167. Total pixels using M3: 623. Difference between M1 and M2 was 2.4°C. Difference between M1 and M3 was 0.6°C. Difference between M3 and M2 was 1.7°C. Refer Section 2.4.2 for details on M1, M2, and M3.