Figure 3.

Multi-lineage differentiation and transcription factor expression of aOBSCs grown on TRG. The aOBSCs were grown as neurospheres, dissociated and seeded on PLO/F-, PEI-, and TRG-coated coverslips for 3–4 days in serum-free medium. They gave rise to the three major cell types of the CNS: neurons (A–C), astrocytes (D–F), and oligodendrocytes (G–I), which were detected using antibodies against β-III tubulin (TuJ1), GFAP, and O4. Graph (J) shows that there was a significant decrease in the total number of cells grown on PEI- (41%) and TRG-coated (33.9%) coverslips compared to PLO/F-coated coverslips. There were no significant differences in the percentage of the three cell fates grown under all three conditions (K). The expression levels of Tubb3 and Gfap transcripts were similar in the three conditions (L). The cell lineage-related transcripts Ngn1, Hes5, and Olig2 were expressed with not significant changes between controls and TRG (M). The bars represent the mean ± SEM (n = 4–13 cultures per condition). Statistical analyses were performed using one-way ANOVA and Tukey’s test, **P < 0.01, ***P < 0.001. Scale bar [shown in (I)] = 100.5 μm.