Figure 6.

TRG supports the morphological maturation of oligodendrocytes derived from aOBSCs. The aOBSC neurospheres were dissociated and seeded in serum-containing medium during 15–18 DIV. Oligodendrocytes were analyzed after immunostaining with an O4 antibody (A–C) investigating further the cells’ morphology, using four parameters shown in the graphs (D–G). There was a significant increase in the cell body perimeter (D) as well as in the number of total branches (F) of cells grown on TRG, compared to PEI. No significant differences were found in other parameters (E,G). The graph bars represent the mean ± SEM (n = 17–32 O4⁺ oligodendrocytes). Statistical analyses were using one-way ANOVA and Tukey’s test. *P < 0.05, ***P < 0.001. Scale bar [shown in (C)] = 50.6 μm.