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Protocols used to differentiate aOBSCs and the assays performed.
| Differentiation condition | DIV | Assays | 2% FBS |
|---|---|---|---|
| Short term (STD) | 3–4 | Multi-lineage differentiation | No |
| Cell interphase SEM | |||
| Medium term (MTD) | 7 | Gene expression | Yes |
| Cell interphase TEM | |||
| Cell survival and morphology | |||
| Long term (LTD) | 15–18 | Cell survival, morphology and synaptic maturation | Yes |
| Cell interphase SEM |
DIV, days in vitro; FBS, fetal bovine serum; SEM, scanning electron microscopy; TEM, transmission electron microscopy.
