. 2016 Dec 6;4:94. doi: 10.3389/fbioe.2016.00094

Table 2.

Primers used for the gene expression profile of differentiating aOBSCs by RT-qPCR.

Gene		Primer (5′–3′)	Size (bp)
Ngn1	Forward	CGATCCCCTTTTCTCCTTTCC	240
Ngn1	Reverse	GTGCAGCAACCTAACAAGTG	240
Hes5	Forward	CAGTCCCAAGGAGAAAAACCG	248
Hes5	Reverse	AGGAGTAGCCCTCGCTGTAGTC	248
Olig2	Forward	CTGGTGTCTAGTCGCCCATC	240
Olig2	Reverse	AGGAGGTGCTGGAGGAAGAT	240
Tubb3	Forward	CAGCGGCAACTATGTAGGGGACTC	148
Tubb3	Reverse	AAAGGCGCCAGACCGAACACT	148
Gfap	Forward	ACCAGCTTACGGCCAACAGTG	204
Gfap	Reverse	CCTCCTCCAGCGATTCAACCT	204
18S	Forward	TCCATTATTCCTAGCTGCGGTATC	111
18S	Reverse	CTCGATGCTCTTAGCTGAGTGTCC	111

The PCR products have the size and sequence of the expected fragments. Ngn1, neurogenin 1; Hes5, hairy and enhancer or split 5; Olig2, oligodendrocyte lineage transcription factor 2; Tubb3, tubulin, beta 3 class III; Gfap, glial fibrillary acidic protein.