Fig. 4.

Effects of PIK-93 on NPSR568-induced increases in Kir2.1 currents. a Effects of NPSR568 with 150 nM PIK-93 pretreatment (10–15 min), on currents recorded from a HEK293T cell transfected with both Kir2.1 and CaR. b Voltage dependence of normalized I. c, d Time courses of NPSR568 (1 μM)-induced averaged changes in currents, showing currents recorded at −115 mV (c) and peak outward currents (d) with 150 nM PIK-93 pretreatment. e, f Quantification of results in c, d, showing the percent increase in currents recorded at −115 mV (e) and peak outward currents (f) induced by 1 μM NPSR568 alone (n = 6) and by 1 μM NPSR568 + 150 nM PIK-93 (n = 3)