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. 2016 Nov 12;468(11):1931–1943. doi: 10.1007/s00424-016-1901-y

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© The Author(s) 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 8 — Effects of U73122 on NPSR568-induced increases in tubby-R332H-cYFP fluorescence on membrane. a Images of tubby-R332H-cYFP fluorescence obtained from cells under control (left panel), during U73122 pretreatment (middle panel), and after (right panel) of 3-μM NPSR568 application with 5 μM U73122 presence all the time. b Time course of averaged changes in tubby-R332H-cYFP fluorescence at the membrane induced by treatment with 3 μM NPSR568, 3 μM NPSR568 + 5 μM U73122, and 5 μM U73122 alone. c Averaged percent increase in fractional fluorescence of tubby-R332H-cYFP at the membrane induced by NPSR568 with and without U73122 pretreatment (n = 12) and by U73122 alone (n = 11)