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. 2016 Dec 6;7:1817. doi: 10.3389/fpls.2016.01817

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Copyright © 2016 López-Castillo, Jiménez-Sandoval, Baruch-Torres, Trasviña-Arenas, Díaz-Quezada, Lara-González, Winkler and Brieba.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of the sulfhydryl reagent MMTS on AtTPI catalytic activity. (A,B) Effect of the addition of increasing concentrations of MMTS on the activity of wild-type and mutant AtTPIs. (A) Illustrates the effect on cTPI activity, and (B) the effect on pdTPI activity. The concentration of MMTS in cTPI varies from 0 to 0.25 μM, whereas the MMTS concentration used for pdTPI is from 0 to 50 μM. The results are expressed as a percentage of the activity with respect to the untreated protein sample. Protein concentrations are indicated.