Table 3.
Accessible thiols present in wild-type and mutants AtTPIs determined by DTNB.
cTPI enzyme version | Accessible thiols (-SH)/monomer | pdTPI enzyme version | Accessible thiols (-SH)/monomer |
---|---|---|---|
WT | 2.0 ± 0.12 | WT | 1.6±0.13 |
C13S | 1.1 ± 0.07 | C15S | 0.1±0.02 |
C218S | 1.0 ± 0.08 | C89S | 0.9±0.1 |
C13S/C218S | 0.9 ± 0.06 | C15S/C89S | 2.0±0.21 |