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. 2016 Dec 6;6:38330. doi: 10.1038/srep38330

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Copyright © 2016, The Author(s)

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Macrophages were infected and exposed to empty BC or to BC-DETC (3.5 or 35 μg/cm²). Cells were assessed for (A) the percentage of infected macrophages and (B) the number of amastigotes per 100 macrophages. (C) The number of viable parasites was evaluated by culture in Schneider medium, free of BC-DETC. Data are from a representative experiment performed in quintuplicate. *p < 0.05, ***p < 0.001.