Figure 6. Treatment of antiserum reduces the bacterial growth and LPS level.
(a) The growth of V. parahaemolyticus. A single colony of the bacterium was inoculated in MLB medium containing heat-inactivated normal mouse serum and mouse antiserum (titer of antibody against LptD was 1:10000) and was grown at 37 °C with shaking at 180 rpm. OD600 values at different time points were determined by spectrophotometry. Statistical significance at each time point is indicated by an asterisk (P < 0.01, n = 3). (b) Silver-stained LPS profiles of V. parahaemolyticus. A single-colony of inoculum cultured in MLB containing heat-inactivated normal mouse serum and mouse antiserum (antibody titer against LptD was 1:10000), respectively. Equal amounts of bacterial cells (2 × 109 CFU) were used to extract LPS using a hot aqueous-phenol method, and the same volume of obtained LPS samples was submitted to electrophoresis on a slab of polyacrylamide gel and was visualized by silver staining.