Figure 2. Quantification of CagL and other Cag proteins in whole cell lysates of several isogenic chromosomal CagL motif deletion mutants (strain SU2).

Bacteria were lysed by sonication (whole cell lysates). Equal protein amounts of each sample (10 μg) were separated on 11.5% SDS gels followed by Western blotting. Proteins CagL, CagI, CagH, Hp antigen, CagA were detected using specific antisera (rabbit α-CagL, 1:20,000; rabbit α-CagI, 1:5,000; rabbit α-CagH, 1:5,000; rabbit α-H. pylori, DAKO, 1:2,500; rabbit α-Hp-CagA, 1:10,000), and matching secondary antisera. CagL, CagI, CagH and CagA amounts in each strain were quantitated by densitometry using ImageJ⁵⁶ (see also Supplementary Figure S7). Invariable Hp antigen bands were used for normalization.