Table 1. Comparative analyses of various chromosomal HP0539/cagL deletion mutants.
H. pylori strains | CagL amount | CagL secretiona | CagI amount | CagH amount | CagA amount | IL-8 induction in AGS cells | CagA translocation |
---|---|---|---|---|---|---|---|
SU2 wt | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
cagL::aphA3 | − | − | + | ++ | ++ | − | n.d. |
CagLΔTSPSA | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
CagLΔNNEM | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
CagLΔRGD | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
CagLΔELFFI | + | + | + | ++ | ++ | + | − |
CagLΔSEDVI | + | + | + | ++ | ++ | ++ | + |
CagLΔFKTYM | + | + | + | ++ | ++ | + | − |
CagLΔLDQNT | − | − | − | ++ | ++ | − | n.d. |
CagLΔAEQQC | + | ++ | ++ | ++ | ++ | ++ | + |
CagLΔGISDK | − | − | + | ++ | ++ | + | n.d. |
CagLΔTASLI | +(+) | ++ | ++ | ++ | ++ | ++ | ++ |
CagLΔRGDΔTASLI | − | + | + | ++ | ++ | ++ | + |
CagLΔTSPSAΔRGD | − | + | + | ++ | ++ | ++ | ++ |
CagLT170K | ++ | + | + | ++ | ++ | ++ | ++ |
The plus and minus signs in each column indicate: [++], [+(+)] normal to slight reduction, [+] clear reduction, and [−] strong reduction of the respective protein or function (column header) in comparison with the parental strain (reference, SU2 wild type [wt]).