Figure 5. Effects of miR-146a on the NF-κB pathway.

(A) SGBS adipocytes were transfected with 20 nM miR-146a mimic or a non-targeting control (NT). 48 h post transfection, cells were stimulated with 10% MacCM or vehicle. Protein was extracted at 0 and 15 min. Western blots using specific antibodies against pIκBα and IκBα were performed with α-tubulin as loading control. One representative out of three independently performed experiments is shown. (B) Densitometric changes are displayed as mean and SEM of all experiments. Statistics: paired t-test, *p < 0.05. (C) Nuclear proteins were isolated and subjected to EMSA to assess the transcriptional activity of NF-κB. One representative of three independently performed experiments is shown.