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. 2016 Dec 6;6:38310. doi: 10.1038/srep38310

Figure 2. PLIN5 overexpression reduces lipolysis and FA oxidation under basal conditions in human primary myotubes.

Figure 2

(A) Representative blot and quantification of PLIN5 protein content in control (Ad-GFP) and PLIN5-overexpressing myotubes (Ad-PLIN5) (n = 3). Pulse-Chase studies using [1-14C] oleate were performed to determine (B) FA release into the culture medium (Ad-GFP = 59 ± 1.8 nmol/3 h/mg protein), (C) FA oxidation (Ad-GFP = 2.22 ± 0.13 nmol/3 h/mg protein), and the rate of incorporation of radiolabeled oleate into (D) TAG, (E) DAG (T0 Ad-GFP = 3.14 ± 0.14 nmol/3 h/mg protein) and (F) intracellular FA content (T0 Ad-GFP = 0.42 ± 0.03 nmol/3 h/mg protein) in control (Ad-GFP) and PLIN5-overexpressing myotubes (Ad-PLIN5). (G) Glycogen synthesis and (H) glucose oxidation were measured in control myotubes (Ad-GFP) and myotubes overexpressing PLIN5 (Ad-PLIN5) using [U-14C] glucose. (I) PDK4 gene expression was measured in control (Ad-GFP) and PLIN5-overexpressing myotubes (Ad-PLIN5). (n = 6) *p < 0.05, **p < 0.01 ***p < 0.001 versus Ad-GFP.