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. 2016 Dec 6;6:38310. doi: 10.1038/srep38310

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Pulse-Chase studies using [1-¹⁴C] oleate were performed to determine the rate of (A,D) incorporation of radiolabeled oleate into TAG and (B,E) oleate oxidation in control myotubes (Ad-GFP) and myotubes overexpressing PLIN5 (Ad-PLIN5) either during (A–C) forskolin (FK) (Ad-GFP CONT = 1.66 ± 0.25 nmol/3 h/mg protein) or (D–E) electrical pulse (EPS) stimulation (Ad-GFP CONT = 6.33 ± 2.05 nmol/24 h/mg protein) (n = 6). Values are expressed in % of Ad-GFP Control (A,B,D,E) and in fold change over control in (C and F). *p < 0.05, **p < 0.01 ***p < 0.001 versus Ad-GFP.