Figure 5. PLIN5 knockdown in mouse skeletal muscle increases lipid oxidation and reduces insulin-stimulated glucose uptake under normal chow diet.

PLIN5 (A) gene expression and (B) protein content measured in control (shNT) and PLIN5 silenced (shPLIN5) mouse tibialis anterior muscle (n = 6). Palmitate (C) and glucose (D) oxidation rate were measured using respectively [U-¹⁴C] glucose or [1-¹⁴C] palmitate in control (shNT) and PLIN5 silenced (shPLIN5) muscle homogenates. Palmitate oxidation (i.e. CO2), acid soluble metabolites accumulation (i.e. ASMs) and total oxidation (i.e. the sum of CO2 release and ASMs accumulation) were measured (n = 6). (E) Insulin-stimulated glucose uptake was determined in control (shNT) and PLIN5 knockdown (shPLIN5) muscles. (n = 7). *p < 0.05, ***p < 0.001 versus shNT.