Skip to main content
. Author manuscript; available in PMC: 2016 Dec 6.
Published in final edited form as: Nat Neurosci. 2016 Mar 21;19(5):668–677. doi: 10.1038/nn.4272

Figure 4.

Figure 4

Poly(GA) proteins cause ubiquitinated proteins to accumulate, decrease the stability of XPC proteins, and sequester XPC into inclusions. (a,b) Immunoblot (a) and densitometric analysis of immunoblots (b) for the indicated proteins to determine their expression in brains of mice expressing GFP, GFP-(GA)50 or GFP-(GA)50-mut (n = 4 per group). Data are presented as mean ± s.e.m. Top left: P < 0.0001, one-way ANOVA; ****P < 0.0001, Tukey’s post hoc analysis. Top right: P = 0.0012, one-way ANOVA; *P = 0.0129 and **P = 0.0010, Tukey’s post hoc analysis. Bottom left: P = 0.1599, one-way ANOVA. Bottom right: P = 0.0820, one-way ANOVA. n.s., not significant. Full-length immunoblots are presented in Supplementary Figure 9. (c) Immunohistochemical analyses of XPC in the cortex of GFP, GFP-(GA)50 or GFP-(GA)50-mut mice. Scale bar, 20 μm. (d) Double immunofluorescence staining for XPC and poly(GA) proteins in the cortex of mice expressing GFP-(GA)50. Scale bar, 5 μm.