FIG. 1.

Histological evaluation of livers from mice gavaged with sesame oil vehicle or 30 µg/kg TCDD every 4 days for 28 and 92 days, as well as following a 36 days recovery period (ie, no treatment) following the final dose at 88 days. A, Representative micrographs of sections stained with hematoxylin and eosin staining (H&E) was used to survey hepatic lesions. Oil Red O (ORO) was used to determine neutral lipid accumulation. PicroSirius Red (PSR) was used to verify collagen deposition. Scale bar represents 50 µm. Portal veins are labeled with the letter v and bile ducts with the letter b, whereas inflammation is denoted by a solid arrow and collagen deposition by a dashed arrow. Quantitation of (B) Oil Red O (ORO) and (C) PicroSirius Red (PSR) staining performed using the Quantitative Histological Analysis Tool (Nault et al., 2015a). Bars represent mean + SEM for at least 3 animals (n = 3–5). Asterisks (*) indicate a significant difference (P ≤ 0.05) between TCDD and vehicle treatment groups determined by two-way ANOVA and Dunnett’s post hoc test for ORO and Student’s t test for PSR.