Skip to main content
. 2016 Sep 9;154(2):392–402. doi: 10.1093/toxsci/kfw176

FIG. 6.

FIG. 6

Representative mass spectra of extracted urine following EH-TBB (100 µmol/kg) administration by gavage to female SD rats. (A) Total ion chromatogram for extracted urine after UPLC separation. (B) Full-scale mass spectrum for peak eluting between 9.1and 9.3 min, m/z of 489.46 Da is consistent with the mass of a TBBA-glycine [M–H]− ion. (C) Full-scale mass spectrum for peak eluting between 9.6 and 9.8 min, m/z of 514.56 Da is consistent with the TBBA-sulfate [M–H]−. (D) Full-scale mass spectrum for peak eluting between 10.7 and 11.2 min, m/z of 481.23 Da is consistent with the TBBA [M + H]− ion with a formic acid adduct (m/z = 481.23 Da); a mass consistent with at TBBA dimer (m/z = 871.56) was formed as well (this was also seen in the TBBA standard, see Supplementary Fig. S3).