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. 2016 Dec 5;15:584. doi: 10.1186/s12936-016-1637-3

Table 1.

Primers for amplification of the region of PfCRT 76

Codon Primer Sequence (5′–3′) Bases Amplicon size
72–97 External forward GACCTTAACAGATGGCTCAC 20 347 bp
External reverse TTTTATATTGGTAGGTGGAATAG 23
Internal forward Biotin-GGTAAATGTGCTCATGTGTTTAAACTTATT 30 241 bp
Internal reverse TTACTTTTGAATTTCCCTTTTTATTTCCA 29
72–76 Pyrosequencing primer AGTTCTTTTAGCAAAAATT 19