Fig. 4.

Effects of E₂ and P₄ treatment in organ culture on meiotic stage. a Total number of oocytes, b percent single oocytes c percent diplotene oocytes and d percent follicles containing diplotene stage oocytes per ovary in control ovaries or ovaries treated with 10⁻⁶ M E₂, 10⁻⁶ M P₄ or 10⁻⁶ M E₂ + 10⁻⁶ M P₄ for 4 days in organ culture. Data are presented as the mean ± SEM. * indicates a significant difference from control ovaries (P < 0.05; n = 5 ovaries per treatment) as determined by one-way ANOVA followed by Dunnett’s multiple comparisons test